Install Apache
Apache2 will now work on most systems with no problems, if it doesn’t for you, section Common Problems might be helpful.
Start by emerging apache2: add “apache2” to your USE flags in make.conf and
emerge apache -av
To start Apache2 run:
/etc/init.d/apache2 start
If you’d like to have have Apache2 start on boot run:
rc-update add apache2 default
Install Mysql (Optional, But highly recommended)
emerge mysql -av
Or if you do not want mysql to be updated automatically with the system,
emerge mysql -1
This will install mysql once without adding its ebuild into world namespace. So next time when you emerge -u world, mysql will be excluded from updating.
Create the initial mysql database
emerge --config mysql
This will also inform you to set the root password of mysql database. If you have more than one version of mysql installed on your machine, please name it sepcifically like =dev-db/mysql-4.1.21
Install Bioperl
The advantage of this approach is it’s stepwise, so it’s easy to stop and analyze in case of any problem.
Download, then unpack the appropriate package. For example:
>gunzip bioperl-1.5.2_102.tar.gz
>tar xvf bioperl-1.5.2_102.tar
>cd bioperl-1.5.2_102
Now issue the build commands:
>perl Build.PL
>./Build test
If you’ve installed everything perfectly and all the network connections are working then you may pass all the tests run in the ‘./Build test’ phase. It’s also possible that you may fail some tests. Possible explanations: problems with local Perl installation, network problems, previously undetected bug in Bioperl, flawed test script, problems with CGI script using for sequence retrieval at public database, and so on. Remember that there are over 800 modules in Bioperl and the test suite is running more than 12000 individual tests, a few failed tests may not affect your usage of Bioperl.
If you decide that the failed tests will not affect how you intend to use Bioperl and you’d like to install anyway, or if all tests were fine, do:
>./Build install
This is what most experienced Bioperl users would do. However, if you’re concerned about a failed test and need assistance or advice then contact bioperl-l@bioperl.org.
To './Build install' you need write permission in the
perl5/site_perl/source area (or similar, depending on your
environment). Usually this will require you becoming
root, so you will want to talk to your systems
manager if you don’t have the necessary privileges.
Cite from http://www.bioperl.org/wiki/Installing_Bioperl_for_Unix.
Now we have a very convenient way to have Gbrowse and all its prerequisites, like latest leased version of bioperl, GD, which were absent from your computer installed.
Download the network installer script: gbrowse_netinstall.pl
I edit gbrowse_netinstall.pl file, change the the gbrowse download mirror to http://jaist.dl.sourceforge.net/sourceforge/gmod/, because the mirror is faster than others, download from China.
use constant SOURCEFORGE_MIRROR1 => 'http://jaist.dl.sourceforge.net/sourceforge/gmod/';
Make sure you have root privilege and run
perl gbrowse_netinstall.pl
Then follow the instruction to complete installation. The default settings are suitable from most circumstances.
The only thing need to be noticed is that the installer uses wrong directories as apache default directories. When the question appears, you should change them to
Apache conf directory? [/usr/local/apache/conf] /etc/apache2/
Apache htdocs directory? [/usr/local/apache/htdocs] /var/www/localhost/htdocs/
Apache cgibin directory? [/usr/local/apache/cgi-bin] /var/www/localhost/cgi-bin/
You may go to the main page of Gbrowse to see more detailed instructions about netinstaller.
Download GBrowse Source from
http://prdownloads.sourceforge.net/gmod
You should be uncompressed and unpacked the source files.
Install GBrowse
Change to the directory which the source files have been uncompressed and unpacked. We will install GBrowse in the default directory “/var/www/localhost/htdocs/” and “/var/www/localhost/cgi-bin/”. Following these command:
perl Makefile.PL APACHE=/var/www/localhost/ CONF=/etc/apache2/ HTDOCS=/var/www/localhost/htdocs/ CGIBIN=/var/www/localhost/cgi-bin/ DO_XS=1
make
make install
Start mysql and apache
/etc/init.d/mysql start
/etc/init.d/apache2 start
Now browse the documentation and example database at http://localhost/gbrowse.
Tao Zhang <forrest_zhang@163.com>.