GMOD

Tripal Tutorial 2011

Tripal

This Tripal tutorial was presented by Stephen Ficklin at the 2011 GMOD Spring Training, March 2011. The most recent Tripal tutorial can be found at the Tripal Tutorial page.

Contents

Introduction to Tripal

What is Tripal

Tripal is a suite of PHP5 modules that bridges the Drupal Content Managment System (CMS) and GMOD Chado.

What is Tripal

Content Management System

Definition From Wikipedia:

A content management system (CMS) is the collection of procedures used to manage work flow in a collaborative environment. These procedures can be manual or computer-based. The procedures are designed to do the following:

In a CMS, data can be defined as nearly anything: documents, movies, pictures, phone numbers, scientific data, and so forth. CMSs are frequently used for storing, controlling, revising, semantically enriching, and publishing documentation. Serving as a central repository, the CMS increases the version level of new updates to an already existing file. Version control is one of the primary advantages of a CMS.

Drupal

Drupal is an open-source freely available CMS with thousands of users and existing sites. Features of Drupal

Drupal website: http://www.drupal.org

Drupal modules: http://www.drupal.org/project/modules

Drupal themes: http://www.drupal.org/project/themes

Chado

The session for Chado was presented yesterday Chado, so no need for introdcutions. However, one thing to remember is that Chado has a modular structure:

Tripal is also modular along these same designations.

Goals of Tripal

Structure of Tripal

Tripal is a collection of modules that integrate with Drupal. These modules are divided into hierarchical categories:

TripalLayers.png

The Tripal Core level contains supportive functionality for all other modules. It contains

The Chado-centric modules provide:

Analysis modules provide

Applications:

Sites Running Tripal

Site Name URL Tripal Version
Pulse Crops Genomics & Breeding <a href=”http://knowpulse2.usask.ca/portal/” class=”external free”  
rel=”nofollow”>http://knowpulse2.usask.ca/portal/</a> SVN version  
Genome Database for Vaccinium <a href=”http://www.vaccinium.org” class=”external free”  
rel=”nofollow”>http://www.vaccinium.org</a> v0.2  
Cool Season Food Legume Database <a href=”http://www.gabcsfl.org” class=”external free”  
rel=”nofollow”>http://www.gabcsfl.org</a> v0.2  
Cacao Genome Database <a href=”http://www.cacaogenomedb.org” class=”external free”  
rel=”nofollow”>http://www.cacaogenomedb.org</a> v0.2  
Fagaceae Genome Web <a href=”http://www.fagaceae.org” class=”external free”  
rel=”nofollow”>http://www.fagaceae.org</a> v0.2  
Citrus Genome Database <a href=”http://www.citrusgenomedb.org” class=”external free”  
rel=”nofollow”>http://www.citrusgenomedb.org</a> v0.2  
Marine Genomics Project <a href=”http://www.marinegenomics.org” class=”external free”  
rel=”nofollow”>http://www.marinegenomics.org</a> pre v0.1  

Resources

The Tripal Sourceforge home site where you can find everything about Tripal: http://tripal.sourceforge.net

GMOD Tripal mailing lists: http://gmod.org/wiki/GMOD_Mailing_Lists

GMOD Tutorials from previous GMOD schools: http://gmod.org/wiki/Tripal

Contributing Organizations

Individuals from these organizations have provided design and coding for Tripal

CUGILogo USLogo WSULogo

Funding

Funding for Tripal has been provided through various grants from the USDA, NSF, in-house funding from the Clemson University Genomics Institute (CUGI), and Clemson’s Cyberinfrastructure and Technology Integration (CITI) group.

Additional Support

Pre-planning

IT Infrastructure

Setup of the prerequisites for Tripal can be the most challenging part depending on the IT infrastructure currently available for your project. Tripal requires a server with adequate resources to handle the expected load and systems administration skills to get the machine up and running, applications installed and everything properly secure. Tripal requires a PostgreSQL databases server, Apache (or equivalent) web server, PHP5 and several configuration options to make it all work. However, once these prerequisites are met, working with Drupal and Tripal are quite easy.

There are three basic ways you could get a Tripal/Drupal/Chado database web server available for your site

  1. Option #1 In-house dedicated servers: You may have access to servers in your own department or group which you have administrative control and wish to install Tripal/Drupal/Chado on these.
  2. Option #2 Institutional IT support: Your institution may provide IT servers and would support your efforts to install a website with database backend.
  3. Option #3 Commercial web-hosting: If options #1 and #2 are not available to you, commercial web-hosting is an affordable option. For large databases you may require a dedicated server.

After selection of one of the options that works best for you you can arrange your database/webserver in the following ways:

  1. Arrangement #1: The database and web server are housed on a single server. This is the approach taken by this course. It is necessary to gain access to a machine with enough memory (RAM), hard disk speed and space, and processor power to handle both services.
  2. Arrangement #2: The database and web server are housed on different servers. This provides dedicated resources to each service (i.e. web and database).

Selection of an appropriate machine

Databases are typically bottlenecked by RAM and disk speed. Selection of the correct balance of RAM, disk speed, disk size and CPU speed is important and dependent on the size of the data. Unfortunately, we have only tested production installations of Tripal on a limited number of server configurations. The best advice is to consult an IT professional who can recommend a server installation tailored for the expected size of your data.

Note: Tripal does require command-line access to the web server with adequate local file storage for loading of large data files. Be sure to check with your service provider to make sure command-line access is possible.

Technical Skills

Depending on your needs, you may need additional Technical support….

Tripal already supports my data, what personnel to I need to maintain it?

Tripal does not yet support all of my data, but I want to use what’s been done and expand on it….?

Why Use Tripal

Tripal does not yet fully support Chado. We are getting closer, but as of now, it is still lacking in many areas… So why use Tripal?

  1. If you want to use Chado
  2. You need a web interface
  3. You need CMS capabilities (distributed content editing, user management, social networking… i.e. Drupal)
  4. You want to contribute to a community effort to help build a tool others can use.
  5. Participate in a community of other database developers using the same technology, confronting similar problems and helping each other.
  6. It is all open-source and free technology!

Pre-Course Setup

By way of information the following steps were performed prior to the course. These steps will not be needed during the class but are here for reference.

Software Used

database management system

Web Prep

Install & Setup PHP

First install php5 using Ubuntu’s apt-get utility:

   sudo apt-get install php5
   sudo apt-get install php5-pgsql
   sudo apt-get install php5-cli
   sudo apt-get install php5-gd

Change some php settings (as root):

   cd /etc/php5/apache2
   sudo gedit php.ini

Set the memory_limit to something larger than 16M (should not exceed physical memory, be conservative but not too much so):

  memory_limit = 2048M;

Now, restart the webserver:

  sudo /etc/init.d/apache2 restart

Do the same for the command-line php.ini:

   cd /etc/php5/cli/
   sudo gedit php.ini

Set the memory limit:

  memory_limit = 2048M;

Install phpPgAdmin

phpPgAdmin is a nice web-based utility for easy administration of a PostgreSQL database. It is not required for successful operation of Tripal but is very useful.

   cd /home/gmod/Documents/Software/tripal/packages
   wget http://downloads.sourceforge.net/phppgadmin/phpPgAdmin-4.2.3.tar.gz?download

As root:

   cd /var/www
   sudo tar -zxvf /home/gmod/Documents/Software/tripal/packages/phpPgAdmin-4.2.3.tar.gz
   sudo ln -s phpPgAdmin-4.2.3/ phppgadmin

Copy the conf/config.inc.php-dist to conf/config.inc.php

   cd phppgadmin/conf
   sudo cp config.inc.php-dist config.inc.php

Set permission for the web user:

   cd /var/www
   sudo chgrp -Rh www-data phppgadmin
   sudo chgrp -Rh www-data phpPgAdmin-4.2.3/

Prepare Apache

Enable the rewrite module for apache. This is useful so that we can use Clean URLs with Drupal. Clean URLs are not required but make the page URLs easier to use. We’ll discuss clean URLs later.

   cd /etc/apache2/mods-enabled
   sudo ln -s ../mods-available/rewrite.load

Next we need to edit the apache configuration file to allow URL rewrites. We can do this by adding the following stanza to the bottom of the file /etc/apache2/sites-available/default:

   <Directory /var/www/>
      RewriteEngine on
      RewriteBase /
      RewriteCond %{REQUEST_FILENAME} !-f
      RewriteCond %{REQUEST_FILENAME} !-d
      RewriteRule ^(.*)$ index.php?q=$1 [L,QSA]
   </Directory>

Now restart your apache again.

sudo /etc/init.d/apache2 restart

Directory Overview

For reference, these are the file system directories where important components of this tutorial are located

   
Web configuration files /etc/apache2
PHP configuration files /etc/php5/apache2 and /etc/php5/cli
Web document root /var/www
Home directory /home/gmod
Tutorial files /home/gmod/Tripal

Database Setup

The postgres database comes pre-installed on the GMOD 2011 VMWare image. Drupal can run on a MySQL or PostgreSQL database but Chado prefers PostgreSQL so that is what we will use for both Drupal and Chado.

With appropriate command-line access and permissions, which we have on this VMWare image) the following command can be used to create a new database user and database for this tutorial.

First, become the ‘postgres’ user:

sudo su - postgres

Next, create the new ‘tripal’ user account. This account will not be a “superuser’ nor allowed to create new roles, but should be allowed to create a database.

createuser -P tripal
  Enter password for new role: gmodamericas2011
  Enter it again:  gmodamericas2011
  Shall the new role be a superuser? (y/n) n
  Shall the new role be allowed to create databases? (y/n) y
  Shall the new role be allowed to create more new roles? (y/n) n

Finally, create the postgres database:

createdb tripal_demo -O tripal

We no longer need to be the postgres user so exit

exit

Install Drupal

Software Installation

Drupal can be freely downloaded from the http://www.drupal.org website. However, to save time, the file has been pre-downloaded onto the VMWare image. You can find the compressed package file here:

/home/gmod/Tripal/drupal-6.20.tar.gz

We want to install Drupal into our web document root (/var/www). We want to be able to do this as our ‘gmod’ user. So, first, let’s set the directory permissions to allow this:

  cd /var
  sudo chgrp gmod www
  sudo chmod g+rw www

In the command above we set the group of the directory to be gmod (our user group) and we gave the directory read and write permissions for the group.

Next, we want to install Drupal. We will use the tar command to uncompress the software:

  cd /var/www
  tar -zxvf /home/gmod/Tripal/drupal-6.20.tar.gz

Notice that we now have a drupal-6.20 directory with all of the Drupal files. We want the Drupal files to be in our document root, not in a ‘drupal-6.20’ subdirectory. So, we’ll move the contents of the directory up one level:

mv drupal-6.20/* ./
mv drupal-6.20/.htaccess ./
mv index.html index.html.orig

Note: It is extremely important the the hidden file .htaccess is also moved (note the second ‘mv’ command above. Check to make sure this file is there

Notice that the last of the three mv commands renames the index.html file and calls it index.html.orig. The index.html file was serving as the home page for the website. Drupal uses an index.php page for it’s home page but the web server has preference for the index.html page. So, we move it out of the way.

Configuration File

Next, we need to tell Drupal how to connect to our database. To do this we have to setup a configuration file. Drupal comes with an example configuration file which we can borrow.

First navigate to the location where the configuration file should go:

  cd /var/www/sites/default/

Next, copy the example configuration that already exists in the directory to be our actual configuration file by renaming it to settings.php.

  cp default.settings.php settings.php

Now, we need to edit the configuration file to tell Drupal how to connect to our database server. To do this we’ll use an easy to use text editor gedit

  gedit settings.php

Find the variable $db_url and set it to this

  $db_url = 'pgsql://tripal:gmodamericas2011@localhost/tripal_demo';

Final directory creation

Finally, we need to create three new directories. The first is the files directory which Drupal uses for storing uploaded files.

  cd /var/www/sites/default
  mkdir files
  sudo chown www-data:gmod files
  sudo chmod g+rw files

The above command creates the directory but sets the owner to be the web server (i.e. www-data) and the group to be gmod, with read/write permissions. This way the web server can write to the directory but so can we.

Also, we need to create two new directories, one for storing module files we’ll be installing and another for themes which we’ll also be installing later:

Now create the modules and themes directory

  cd /var/www/sites/all
  mkdir modules
  mkdir themes

Compatibility with other tools

We want to ensure that our Drupal installation doesn’t interfere with other web-based tools, such as GBrowse. We need update a setting in the .htaccess file that came with Drupal which instructs the web server to look for both index.php and index.html files when serving pages.

Use ‘gedit’ to modify the /var/www.htaccess file.

   cd /var/www
   gedit .htaccess

Locate the line DirectoryIndex and change it to mach the following:

  DirectoryIndex index.php index.html

Web-based Steps

Navigate to the installation page of our new web site http://localhost/install.php

Install1

Click the link in the middle section that reads Install Drupal in English

Tripal_Install2

When the progress bar shows completing the page will switch to a configuration page with some final settings.

Tripal-install3

Set the following

Now, click the Save and Continue button. You will see a message about unable to send an email. This is safe to ignore as email capabilities are not fully enabled on this VMWare image. Now, your site is enabled. Click the link Your new site:

Tripal_Install4

Drupal Cron Entry

The last step for installing Drupal is setting up the automatted Cron entry. The Drupal cron is used to automatically execute necessary housekeeping tasks on a regular interval. Cron is a UNIX facility for scheduling jobs to run at specific intervals.

Drupal itself requires an entry in the crontab to function. To edit the cron launch the crontab editor:

  sudo crontab -e

A word on text editors such as nano.

Add this line to the crontab

  0,30 * * * * /usr/bin/wget -O - -q http://localhost/cron.php > /dev/null

Now save the changes. We have now added a UNIX cron job that will occur every 30 minutes that will execute the cron.php script and cause Drupal to perform housekeeping tasks.

Explore Drupal

User Account Page

All users have an account page. Currently, we are logged in as the administrator. The account page is simple for now. Click the My account link on the left sidebar. You’ll see a brief history for the user and an Edit tab. Users can edit their own information using the edit interface:

ExplorDrupal1

Creating Content

Creation of content in Drupal is very easy. Click the Create content link on the left sidebar.

ExplorDrupal2.png

You’ll see to content types that come default with Drupal: Page and Story. Here is where a user can add simple new pages to the website without knowledge of HTML or CSS. Click the Page content type to see the interface for creating a new page:

ExploreDrupal3

You’ll notice at the top a Title field and a Body text box. All pages require a title and typically have some sort of content entered in the body. Additionally, there are other options that allow someone to enter HTML if they would like, save revisions of a page to preserve a history and to set authoring and publishing information.

ExploreDrupal4

Let’s create two new pages. A Home page and an About page for our site. First create the home page and second create the about page. Add whatever text you like for the body.

Site Administration

Content Management

There are many options under the Administer link on the left sidebar. Here you can manage the site setup, monitor and control content, manage users and view reports.

DrupalAdmin1

We will not explore all of the options here but will visit a few of the more important ones for this tutorial. First, click the Content Management link on the left sidebar. You’ll see different options.

DrupalAdminContent

Click the Content link. The page shows all content available on the site. You’ll see the two pages we created previously:

DrupalContent

You’ll also notice a set of drop down boxes for filtering the content. For sites with many different content types and pages this helps to find content. You can use this list to click to view each page or to edit.

Site Building

Modules

Click the Site Building link on the let sidebar under the Administer link. You’ll see several new menu options: Blocks, Menus, Modules and Themes. First click Modules

AdminModules

Here is where you will see the various modules that make up Drupal. Take a minute to scroll through the list of these and read some of the descriptions. The modules you see here are core modules that come with Drupal. Those that are checked come pre-enabled. Those that are not checked we will need to install. For this tutorial we will need two additional modules that are not yet installed. Locate the modules Path and Search and check the box next to each of those. Scroll to the bottom and click ‘Save configuration’.

The Path and Search modules are now installed. The Search module enables site-wide searching capabilities for our site and the Path module enables alternative naming of page URLs (we will discuss later).

Module Installation

We can install new extension modules which we will need later. For this workshop we have several modules that we will need to install but which do not yet appear in the list of modules. To do this, we must follow these steps:

  1. Locate and download the extension modules from the Drupal website
  2. Unpack the module in the /var/www/sites/all/modules directory
  3. Check for a README.txt or INSTALL.txt for any further instructions for installation of the module
  4. Return the the Drupal ‘Administer’ -> ‘Site Building’ -> ‘Modules’ page and enable the module.

For an example, let’s install the Views module needed for this workshop. The views module has been pre-downloaded and saved in the /home/gmod/Tripal directory.

First, let’s look and see what’s there

  ls /home/gmod/Tripal

TripalhomeDir

There are several .tar.gz files. These are all Drupal modules we’ll be installing for this workshop. To install the Views module go to our modules directory, unpack the file.

  cd /var/www/sites/all/modules
  tar -zxvf /home/gmod/Tripal/views-6.x-2.12.tar.gz

Check the README for additional installation instructions

  cd views
  ls
  less README.txt

Use the space-bar to scroll through the README.txt file. Hit the ‘q’ key to quit

There are not other installation steps besides what we’ve done. So return to the AdministerSite BuildingModules page and enable the module.

DrupalViews

Notice that the Views package provided three different related modules and they all appear under a Views category.

EXERCISE #1

 Install the Chaos tools modules on your own.  The packages is found here:

 /home/gmod/Tripal/ctools-6.x-1.8.tar.gz

 Enable these modules under the Chaos tool suite category:
 * Chaos tools
 * Custom content panes
 * Page Manager
 * Views content panes

Next, we need the JQuery UI module. We will do this one together as it has a dependency we must install. The package is found here: /home/gmod/Tripal/jquery_ui-6.x-1.4.tar.gz

  cd /var/www/sites/all/modules
  tar -zxvf /home/gmod/Tripal/jquery_ui-6.x-1.4.tar.gz

Check the README for additional installation instructions

  cd jquery_ui
  ls
  less README.txt

The installation file indicates we need to install the JQuery UI package before we can eanble this module. We must first get this package from online.

Here is a quick command for downloading this file

  cd /var/www/sites/all/modules/jquery_ui
  wget http://jquery-ui.googlecode.com/files/jquery.ui-1.6.zip

Now unzip the package and rename it according to the instructions

  unzip jquery.ui-1.6.zip
  mv jquery.ui-1.6 jquery.ui

Now, return to our Drupal website and enable the Jquery UI module. It can be found under the User Interface category.

EXERCISE #2

 Now install the module package Panels found here  /home/gmod/Tripal/panels-6.x-3.9.tar.gz

 Enables these modules under the Panels category:
 * Mini panels
 * Panel nodes
 * Panels

EXERCISE #3

 For this last exercise, we need to install the Content-Construction Toolkit (CCK) module.
 For this exercise we will download the file from the Drupal site: http://drupal.org/project/cck.
 Be sure to download the 6.x-2.9 version.  Follow the same installation steps as performed
 previously.  Enable all modules under the CCK category.

For reference, the modules installed above can be found here:

Themes

Next, click the Themes link under AdministerSite Building on the left sidebar.

DrupalThemes

Here, you’ll see a list of themes that come with Drupal by default. If you scroll down you’ll see that one theme named Garland is enabled and set as default. The current look of the site is using the Garland them. Change the them by checking the Enable checkbox and the default radio button for the Pushbutton theme and then clicking Save configuration. Now you’ll see that the theme has changed.

DrupalThemes2

Theme Installation

Drupal allows us to install new themes. Installation of themes involves these steps:

  1. Locate and download a theme from the Drupal website (http://www.drupal.org/themes)
  2. Unpack the theme in the /var/www/sites/all/themes directory
  3. Return to the Drupal AdministerSite BuildingThemes page and enable the theme

For an example, the Sky theme is available in the home directory on this VMWare image. We’ll install that.

  cd /var/www/sites/all/themes
  tar -zxvf /home/gmod/Tripal/sky-6.x-3.11.tar.gz

This should unpack the theme for us. Now, navigate to AdministerSite BuildingThemes and enable the ‘Sky’ theme:

DrupalThemes3

The sky theme was obtained at this address: http://drupal.org/project/sky

Blocks

Blocks in Drupal are used to provide content in regions of a Drupal theme. For example, navigate to AdminsterSite BuildingBlocks.

You’ll see that regions of the theme have been identified. Within the Sky theme you can see the regions with dashed lines around them. Also, you’ll see a list of available blocks. You can select where blocks will appear by selecting the region in the drop down list. Blocks may also be hidden, if desired, by selecting <none> in the dropdown.

DrupalBlocks

Exercise #4

 Take time to turn on and off blocks to see where they appear.  Re-arrange blocks
 by dragging and dropping the cross-hairs beside each one.  Be sure to leave the
 blocks in the configuration shown in the image below finished:

DrupalBlocks2

Drupal provides an interface for working with menus, including adding new menu items to an existing menu or for creating new menus. For the exercise in the Blocks section above we added the Primary links menu to the Content top section of the Sky theme. To view the Primary links menu, navigate to AdministerSite buildingMenus.

DrupalMenus1

Select the menu Primary links. You’ll see it currently has no item.

DrupalMenu2

As a demonstration for working with menus we’ll add two menu items for the Home and About pages we created earlier. To do so, click the Add item tab. You will see a form for providing information about the menu item to be added.

DrupalMenuAdd

The first field is the path. We need to find the path for our home page.

Exercise #5

 Open new browser tabs or windows and find the About page
 and the Home page we created earlier.

The path for a page can be found in the address bar for the page. In Drupal pages of content are generally referred to as nodes. So, in the address bar for our home page you’ll see the address is http://localhost/node/1. Our about page should be http://localhost/node/2 (i.e the first and second pages we created).

The path for each of these nodes is simply node/1 and node/2. Returning to our tab where we are adding a menu item, enter the path node/1. We will set the fields in this ways:

The settings above will give the menu link a title of Home and put it on the Primary Links menu. We now have a Home menu item in the top just under the header, and our Home menu item now appears in the list of menu items for the Primary Links menu

DrupalMenu4

Exercise #6

 Add a second menu item for our about page and arrange them so that our Home
 link appears first and the About link appears second.
URL Path

As mentioned previously, the URL paths for our pages have node/1 and node/2 in the address. This is not very intuitive for site visitors. Earlier we enabled the Path module. This module will allow us to set a more human-readable path for our pages.

To set a path, click on our new About page in the new menu link at the top and click the Edit tab. Scroll to the bottom of the edit page and you’ll see a section titled URL path setting. click to open this section.

DrupalAboutEdit

Since this is our about page, we simply want the URL to be http://localhost/about. To do this, just add the word about in the text box. You will now notice that the URL for this page is no longer http://localhost/node/2 but now http://localhost/about. Although, both links will still get you to our About page.

Exercise #7

 Set a path of 'home' for our home page.
Site Configuration

There are many options under the AdministerSite configuration page. Here we will only look at one of these at the moment–the Site Information page.

DrupalSiteInfo

Here you will find the configuration options we set when installing the site. You can change the site name, add a slogan, mission and footer text to the. Towards the bottom there is a text box titled Default front page. This is where we can tell Drupal to use our new Home page we created as the first page visitors see when the view the site. In this text box enter the text node/1. This is the address of our home page. We must use the node number here and not our new URL pat of ‘home’ that we just created. Let’s change the name of our site from Tripal demo to Fly Genes Database and add a slogan: Resources for Fly Genomics.

Now, click the Save configuration button at the bottom. You’ll see our site name has changed at the top. Also, if we click the logo image at the top of the site and it will take you to the front page with our new home page appearing.

Theme Configuration

Here we return to theming. There are several configuration options that are available to help customize the theme for your site. These can be found by navigating to the AdministerSite BuildingThemes page and clicking the Configure tab near the top.

Appearing under the Configure link will be small menu with a listing of every theme we have enabled. You should see the Sky theme at the end of this list. Click that theme because that is the one we are using and want to configure:

DrupalSkyTheme

Here you can turn on and off the presence of the logo, site name, slogan, mission statement, etc. For this particular theme we can also adjust background colors and images, link colors, font style and size, and more. Notice when we added a slogan in the previous step that it did not appear anywhere on the site. To make it appear, check the box next to Slogan.

We also want to add a new logo for our site. Since we’ll be loading fly data we want to add a picture of a fruit fly as our logo. We will use the following image:

Drosophila.png

This image was taken from Wikimedia Commons (http://commons.wikimedia.org/wiki/File:Drosophila.png). Right-click on this image and download it somewhere on your machine. Next, return to our Drupal theme configuration page and use the Logo file upload interface on the page to upload this file as our logo.

Also set the following for the theme:

Then, click the Save Configuration button at the bottom. We now have a new logo and our slogan appears.

DrupalLogo

User Accounts

We will not discuss in depth the user management infrastructure except to point out:

Tripal Tutorial

Installation

We’re going to use the development version from SVN because it has so many more features that the current v0.2. A new version, v0.3 will be released soon and will have many of these features we’ll be using in the development version. A recent version from SVN was downloaded to he /home/gmod/Tripal/tripal-svn directory. The following command was used to do this. You do not need to perform this step as it has been done for you:

svn co https://gmod.svn.sourceforge.net/svnroot/gmod/tripal/trunk
mv trunk tripal-svn

However, since this version of Tripal was downloaded to the VMWare image there have been further updates which we want to include for this workshop. To get those we need to update the SVN. We can do this simply by issuing the ‘svn update’ command:

cd /home/ficklin/gmod/Tripal/tripal-svn
svn update

To install Tripal we need to do the same as we did for the other modules, with the exception of unpacking the files. The files are already decompressed. So, let’s just copy them to our Drupal modules directory:

cp -R tripal_* /var/www/sites/all/modules
cp -R theme_tripal /var/www/sites/all/themes

Note:: typically, one would download the Tripal package and uncompress it as we did with the other modules we installed. But since we’re using SVN we just copy it over.

There are no special instructions for installing Tripal other than copying the files so we will now navigate to the AdministerSite BuildingModules page to install Tripal. Here you’ll see a long list of modules under two categories: Tripal and Tripal Analyses.

TripalModules

Enable the module Tripal core only. There is now a Tripal Management link under Administer with several sub items:

TripalManagement1

Install Chado

The first thing we want to do is install Chado. If Chado is already installed in another database then Tripal can use that Chado as well. However, for better compatibility with all functionality of Drupal it is best if Drupal and Chado live in the same database. Instructions for integrating Tripal with an external Chado database are provided in the User’s Guide but will not be shown in this workshop.

To install Chado, simply navigate to the page AdministerTripal ManagementInstall Chado v1.11, read the warning message and click Install Chado when ready.

TripalInstallChado

A message will then be given that says "Job 'Install Chado' submitted. Click the jobs page for status". If we then click the link jobs page we can then see our job in the list:

TripalJobs1

We will continue our installation of Chado in the next section.

Jobs Management

The jobs management subsystem allows modules to submit long-running jobs, on behalf of site administrators or site visitors. Often, long running jobs can time out on the web server and fail to complete. The jobs system runs separately in the background using the command-line on an automated schedule but jobs are submitted through the web interface by users.

Tripal provides two API function calls that allows any module to submit a job to the queue

So, in the example above we now see a job for installing Chado. The job view page provides details such as the name of the job, The user who submitted the job, dates that the job was submitted and job status.

Jobs in the queue can be executed in two ways:

When we installed Drupal we installed a Cron job to allow the software to run housekeeping tasks on a regular bases. Tripal needs a cron entry as well to allow for regular execution of jobs in the queue. We will need to add a second cron entry:

   sudo crontab -e

A word on text editors such as nano.

Add this line to the crontab

   0,15,30,45 * * * * (cd /var/www; php ./sites/all/modules/tripal_core/tripal_launch_jobs.php administrator ) > /dev/null

This entry will run the Tripal cron every 15 minutes as the administrator user. For this workshop we do not want to wait 15 minutes at the most to execute our jobs. So, we’ll run the jobs in the queue manually. We will execute the same command that we used in the cron above:

  cd /var/www; php ./sites/all/modules/tripal_core/tripal_launch_jobs.php administrator

We should now see that Chado is being installed!

TripalChadoInstalled

Also, we see that the job has completed when refreshing the jobs management page:

TripalChadoInstalled2

PhpPgAdmin

A nice database management tool is freely available for managing a PostgreSQL database. It is currently installed on the VMWare image and is accessible at this URL: http://localhost/phppgadmin/

Let’s look at our Chado and Drupal databses using phpPgAdmin by first logging in with the username gmod and password gmodamericas2011. When the tool opens you’ll see several databases listed down the left-hand side. Click the tripal_demo database to open the tree.

TripalPhpPgAdmin1

Under the tripal_demo database you’ll see several different schemas:

The chado, frange, genetic_code and so schemas were all installed by our Chado installation. The public schema is used by Drupal. All of the chado tables that we will be using are housed in the ‘chado’ schema and all tables for Drupal are in the ‘public’ schema.

Open the chado schema look at the Chado tables. Then open the public schema and take a look at the Drupal tables

The Chado database:

TripalPhpPgAdmin2

The Drupal database

TripalPhpPgadmin3

Install Additional Tripal Modules

Now that we have the core module installed and the Chado database installed and ready we can install the other Tripal modules.

Note: For this workshop we will not be using all of the modules available for Tripal, only those related to the Chado feature module.

Return to the AdministerSite buildingModules page and turn on the following Tripal modules in the order specified:

  1. Tripal DB
  2. Tripal CV
  3. Tripal Organism
  4. Tripal Analysis
  5. Tripal Feature

Notice that for some of these modules messages appear after installation indicating directories being created and Materialize views (we will discuss these in a bit).

Now, when you visit the Administer’ → Tripal Management page you will see several more administrative options available to you.

Install the Tripal Theme

Tripal provides a theme for Drupal, but it is designed to be a base theme. This way the site can use any theme you want, but Tripal can still provide some theming support for the data components it generates. To enable the Tripal theme, Navigate to the AdminsterSite BuildingThemes page and click the checkbox to Enable the Tripal theme. DO NOT click the default radio button for the Tripal theme. We do not want it to be default. The Sky theme should remain as default.

TripalBaseTheme1

Next we have to inform our Enabled them (a.k.a the Sky theme) that it should use Tripal as it’s base theme. To do this, we need to edit the .info file in the Sky theme directory.

   cd /var/www/sites/all/themes/sky
   gedit sky.info

Scroll to the bottom of the file and add the following line

   base theme = tripal

Materialized Views

Chado is very efficient as a data warehouse but queries can become slow depending on the number of table joins and amount of data. To help simplify and speed these queries, materialized views were introduced into Chado by the GMOD folks.

A materialized views will take an SQL query like this

   SELECT DISTINCT CVT.name,CVT.cvterm_id, CV.cv_id, CV.name
   FROM {cvterm_relationship} CVTR
     INNER JOIN cvterm CVT on CVTR.object_id = CVT.cvterm_id
     INNER JOIN CV on CV.cv_id = CVT.cv_id
   WHERE CVTR.object_id not in
        (SELECT subject_id FROM {cvterm_relationship})

And turn it into this:

     SELECT * FROM cv_root_mview WHERE cvterm_id = 100

For this to work a table named cv_root_mview is created and populated with the results of the larger query. The query on the materialized view is there more simple and faster. A side effect, however is redundant data, with the materialized view becoming stale if not updated regularly.

Tripal provides a mechanism for updating these materialized views. These can be found on the AdministerTripal ManagementMaterialized Views page.

TripalMV1

Here we see two materialized views. These were installed automatically by the Tripal CV and Tripal Feature modules. We need to update these and populate them with data. To do so, click the Update button for the first one. This will add a job to our jobs management system to update the view. Now, return to the Materialized Vies page and click to update the other view as well.

So, let’s run these two jobs:

  cd /var/www; php ./sites/all/modules/tripal_core/tripal_launch_jobs.php administrator

You can now see that both views are up-to-date on the Materialized Views Page

TripalMV2

Controlled Vocabularies: Installing CVs

Before we can proceed with populating our Chado table with genomic data we must first load some Ontologies. To do this, navigate to AdministerTripal ManagementControlled Vocabularies. You’ll see a page describing the purpose of the module and how to use it (right now just a shell). On the left hand menu find the link for ‘Add/Update Ontology With OBO File’ and click that. You’ll see the following page:

TripalAddOntology

The Ontology loader will allow you to select a pre-defined ontology from the drop down list or allow you to provide your own to be loaded. If you were to provide your own, you would give the remote URL of the OBO file or provide the full path on the local web server where the OBO file is located. In the case of the file residing remotely, Tripal first download and the parse the OBO file for loading. If you do provide your own OBO file it will appear in the saved drop down list for loading of future updates to the ontology.

For this workshop, need to install these ontologies:

  1. Chado feature properties
  2. Relationship ontology
  3. Sequence ontology

Do so by selecting one and clicking the Submit button. Repeat this process for each of the three ontologies. You’ll notice each time that a job is added to the jobs subsystem.

Now manually launch these jobs

  cd /var/www; php ./sites/all/modules/tripal_core/tripal_launch_jobs.php administrator

Note: Normally, we would also want to load the Gene Ontology. However, this process will take several hours which we do not have time for in the lecture. Unfortunately, this means we will not be able to demonstrate the GO analysis module in this workshop.

Organism Page

What if Our Organism is Already in Chado?

Now that we have Chado loaded and populated we would like to create a home page for our species. Chado comes pre-loaded with a few species already, so we will check to see if our organism is already present. To do this navigate to Administer -> Tripal ManagementOrganismsConfiguration

TripalOrganisms

This configuration page has several different options. We will discuss two of these here. The first is the top section labeled Sync Organisms. In this section is a list of organisms. These are the organisms that come by default with Chado. Fortunately our Organism is already in the list: Drosophila melenogaster

We need to inform Drupal that we have data in Chado that we would like a web page for. This is what we call Syncing. We need to sync Drupal and Chado so that Drupal knows about our organism. To do this, click the check box next to Drosophila melenogaster and then click the Submit Sync Job.

As usual we want to run this job manually:

  cd /var/www; php ./sites/all/modules/tripal_core/tripal_launch_jobs.php administrator

Now that our organism is synced we should have a new page for Drosophila melenogaster. To find out, click the Organisms menu item at the top of the page. This menu item was automatically added when we installed the Tripal Organism module. On this page we see a list of organisms that have been “synced” with Drupal and Chado. There should only be our the one we just synced.

TripalOrganisms2

Now if we click the ‘fruitfly’ link it should take us to our new organism page:

TripalOrganisms3

This page, however is a bit empty. We need to add some details. Click the Edit tab towards the top of the page. Notice two if the fields are missing content: the description and the organism image

TripalOrganism4

For the description add the following text (taken from wikipedia: http://en.wikipedia.org/wiki/Drosophila_melanogaster):

“The genome of D. melanogaster (sequenced in 2000, and curated at the FlyBase database) contains four pairs of chromosomes: an X/Y pair, and three autosomes labeled 2, 3, and 4. The fourth chromosome is so tiny that it is often ignored, aside from its important eyeless gene. The D. melanogaster sequenced genome of 165 million base pairs has been annotated[17] and contains approximately 13,767 protein-coding genes, which comprise ~20% of the genome out of a total of an estimated 14,000 genes. More than 60% of the genome appears to be functional non-protein-coding DNA involved in gene expression control. Determination of sex in Drosophila occurs by the ratio of X chromosomes to autosomes, not because of the presence of a Y chromosome as in human sex determination. Although the Y chromosome is entirely heterochromatic, it contains at least 16 genes, many of which are thought to have male-related functions.”

For the image, download this image below and upload it using the interface on the page.

Dmel.jpg

Save the page. Now we have a more informative page:

TripalOrganism5

What if Our Organisms Isn’t Present in Chado?

If we have an organism that is not already present in Chado, we can easily add one using the Create Content page. You can find this link on the left side bar navigation menu. The Create Content page has many more content types than when we first saw it. Previously we only had Page and Story content types. Now we have three additional content types added by the Tripal Analysis, Organism and Feature modules.

DrupalCreateContent

To add a new organism simply click the Organism link and and fill in the fields.

TripalOrganism6

Excercise #8

 Create a new organism of your choice.   Once complete, click the
 Organism menu item at the top to see it in the list.

Loading Data

Review of the Data

Now that we have our organism ready, we can to being loading feature data. For this workshop only a few genomic features from the genome of Drosophila melenogaster (obtained from FlyBase) have been selected. This is to ensure we can move through the demonstration rather quickly. But Tripal can support very large datasets. Also, we are not loading the entire compendium of data for these genomic features. Tripal does not have loaders or visualization pages for all of the data types available on FlyBase.

Below is a summary of the data types Tripal can currently handle for genomic features:

  1. Genomic loci or features (e.g. genes, mRNA, CDS, EST, contig, or any other type in the Sequence Ontology)
  2. Feature relationships (e.g. mRNA part_of gene, protein derived_from mRNA).
  3. Feature synonyms
  4. External Database cross-references for features
  5. Feature localizations (e.g. genes are located on chromosomes or scaffolds)
  6. Feature properties

Also display of analyses results are also available for these methods:

  1. Blast
  2. KEGG
  3. InterProScan
  4. Gene Ontology
  5. Unigene

We will demonstrate all of the above with the exception of the Gene Ontology viewer (because the GO takes too long to load for this course) and the Unigene (we are not building a unigene set).

Loading a GFF3 File

GFF3 files can be parsed and data loaded into Chado using the GFF3 loader that now comes with Chado. The GFF loader supports these operations:

As of the SVN version it does not yet support (but will as time progesses):

A GFF3 file containing the genes and mRNA sequences we will be using can be downloaded here: Flybase-tripal-demo.gff (Aside from filtering the original FlyBase GFF file, no alterations have been made to this file).

Note: The file has a .txt extension because the wiki would not allow a .gff extension.

Right-click and download this data into the directory /home/gmod/Tripal

Now, navigate to AdministerTripal ManagementFeaturesImport a GFF3 file.

TripalGFF3Import

Here we will simply choose the organism to which the GFF3 file describes, enter the path on the file system where our GFF file resides and select the appropriate import options.

Enter the following values

Click the Import GFF3 button when complete. You’ll notice a job was submitted to the jobs substem. Now, to complete the process we need the job to run. We’ll do this manually:

  cd /var/www; php ./sites/all/modules/tripal_core/tripal_launch_jobs.php administrator

Feature Pages

Syncing Features

Just as we had to sync our organisms, we must also do the same for our genomics features. Loading of the GFF file in the previous step has populated the feature tables of Chado for us, but now Drupal must know about these features. Similar to the Organism Configuration page there is also a Feature Configuration page. This can be found by navigating to AdministerTripal ManagementFeaturesConfiguration.

TripalFeaturesConfig

The first text box is for specifying internal ID prefix. This is used to form a unique numeric identifier for each feature in your site. The number is prefixed with the value chosen here. Enter a value of ‘FGD’ for Fly Genes Database.

The second text box is for specifying the types of features to sync with Drupal. You should enter the Sequence Ontology terms for the features that should have pages on the site. In our case, we want gene and mRNA pages. Features of these types were present in our GFF file. So, add the SO terms ‘gene’ and ‘mRNA’ to the this box.

Now, click the Save configuration button at the bottom of the page to save the changes.

Next, we want to sync the ‘gene’ and ‘mRNA’ features with Drupal. To do this, scroll down until you find the Sync Features section. click the Sync All Features button. A job is then added to the jobs management system which we need to manually run.

  cd /var/www; php ./sites/all/modules/tripal_core/tripal_launch_jobs.php administrator

Our features are now synced:

TripalFeatureSync

Exercise #9

 Find the feature pages we just synced.   We previously discussed one way to find content.

Manually Adding a New Feature

In the same way that we created an organism using the Create content page, we can also add a new feature as well. We will not review how to do this as the steps are the same as for creating an organism. Also, it is not practical to add all features in this way, but this interface can be useful for cases where a feature may have been forgotten and needs to be easily added.

Loading FASTA files

Locate the gene page for the gene named ‘CG40042’. Scroll through the page. Notice, we can see the feature details, synonyms, external database references, locations for this feature and relationships that this feature has. We are missing the sequence information. When we loaded the GFF file there was no sequence data (although our GFF loader currently doesn’t handle FASTA sequences in the GFF file… it will in the future). So, we need to add the sequence data. We also want to add peptide information as well.

To do this, we will load three FASTA files which you can download from these links:

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