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Load a custom ontology in Chado
Howto load a custom ontology in Chado
We have an ontology that we want to load into Chado. The process to follow is described in the INSTALL.Chado document.
If you already have the desired ontology files locally, you can execute a command for each file to load it. Note again that the Relationship Ontology is required before all others, and the the Sequence Ontology (SO) is absolutely required for proper functioning of the database. The commands to load an ontology are: $ go2fmt.pl -p obo_text -w xml /path/to/obofile | \ go-apply-xslt oboxml_to_chadoxml - > obo_text.xml This creates a chadoxml file of the obo file - then execute: $ stag-storenode.pl \ -d 'dbi:Pg:dbname=$CHADO_DB_NAME;host=$CHADO_DB_HOST;port=$CHADO_DB_PORT' \ --user $CHADO_DB_USERNAME --password $CHADO_DB_PASSWORD obo_text.xml If you have other ontology format files, the commands are similar; consult the documentation for go2fmt.pl and go-apply-xslt for your file format.
Here we explained with more detail.
We have an ontology stored in an obo file. As an example we can try to load the following ontology.
format-version: 1.2 default-namespace: library_type [Term] id:internal_go_lib:000001 name: library_type [Term] id: internal_go_lib:000002 name: cDNA library definition: a collection of complementary DNA fragments is_a: cmv_internal_go_lib:000001 [Typedef] id: internal_go_lib:is_a name: is_a definition: The basic subclassing relationship [Typedef] id: internal_go_lib:disjoint_from name: disjoint_from definition: Indicates that two classes are disjoint [Typedef] id: internal_go_lib:instance_of name: instance_of definition: Indicates the type of an instance [Typedef] id: internal_go_lib:inverse_of name: inverse_of definition: Indicates that one relationship type is the inverse of another [Typedef] id: internal_go_lib:union_of name: union_of definition: Indicates that a term is the union of several others [Typedef] id: internal_go_lib:intersection_of name: intersection_of definition: Indicates that a term is the intersection of several others [OBO:defs]
The name of the file should match the database that will go in the db table (although I'm not sure about that). I've named this file as internal_go_lib.
To load the ontology into chado we have to create a Chado xml file. There are two options to do it:
- go2fmt.pl to create a go xml and go-apply-xslt to transform it to chado xml (as described in the install).
- go2chadoxml to go from obo to chado xml in one go.
Let's do it with go2chadoxml
$ go2chadoxml internal_go_lib > internal_go_lib.chado.xml
We have obtained an xml file ready to be loaded into chado.
Loading into Chado
Now we can load the xml file into chado with the stag-storenode.pl command.
stag-storenode.pl -d 'dbi:Pg:dbname=chado_test;host=localhost;port=5432' --user chado_user --password chado_pass internal_go_lib.chado.xml
Prior to that loading in the cv table I had added library_type and internal_go_lib in the db table. I don't know if that's required, I guess that stag-storenode.pl will do it for you anyway.