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Load a custom ontology in Chado

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Howto load a custom ontology in Chado

We have an ontology that we want to load into Chado. The process to follow is described in the INSTALL.Chado document.

If you already have the desired ontology files locally, you
can execute a command for each file to load it.  Note again that
the Relationship Ontology is required before all others, and the
the Sequence Ontology (SO) is absolutely required for proper
functioning of the database.  The commands to load an ontology are:

      $ -p obo_text -w xml /path/to/obofile | \
            go-apply-xslt oboxml_to_chadoxml - > obo_text.xml

This creates a chadoxml file of the obo file - then execute:

     $ \
     -d 'dbi:Pg:dbname=$CHADO_DB_NAME;host=$CHADO_DB_HOST;port=$CHADO_DB_PORT' \
     --user $CHADO_DB_USERNAME --password $CHADO_DB_PASSWORD obo_text.xml

If you have other ontology format files, the commands are similar;
consult the documentation for and go-apply-xslt for your
file format.

Here we explained with more detail.

The ontology

We have an ontology stored in an obo file. As an example we can try to load the following ontology.

format-version: 1.2
default-namespace: library_type

name: library_type

id: internal_go_lib:000002
name: cDNA library
definition: a collection of complementary DNA fragments
is_a: cmv_internal_go_lib:000001

id: internal_go_lib:is_a
name: is_a
definition: The basic subclassing relationship

id: internal_go_lib:disjoint_from
name: disjoint_from
definition: Indicates that two classes are disjoint

id: internal_go_lib:instance_of
name: instance_of
definition: Indicates the type of an instance

id: internal_go_lib:inverse_of
name: inverse_of
definition: Indicates that one relationship type is the inverse of another

id: internal_go_lib:union_of
name: union_of
definition: Indicates that a term is the union of several others

id: internal_go_lib:intersection_of
name: intersection_of
definition: Indicates that a term is the intersection of several others [OBO:defs]

The name of the file should match the database that will go in the db table (although I'm not sure about that). I've named this file as internal_go_lib.

Chado xml

To load the ontology into chado we have to create a Chado xml file. There are two options to do it:

  • to create a go xml and go-apply-xslt to transform it to chado xml (as described in the install).
  • go2chadoxml to go from obo to chado xml in one go.

Let's do it with go2chadoxml

$ go2chadoxml internal_go_lib > internal_go_lib.chado.xml

We have obtained an xml file ready to be loaded into chado.

Loading into Chado

Now we can load the xml file into chado with the command. -d 'dbi:Pg:dbname=chado_test;host=localhost;port=5432' --user chado_user --password chado_pass internal_go_lib.chado.xml

Prior to that loading in the cv table I had added library_type and internal_go_lib in the db table. I don't know if that's required, I guess that will do it for you anyway.