Difference between revisions of "GBrowse 2.0 Prerequisites"
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You're done installing prerequisites! You can now go back to the [[GBrowse 2.0 Install HOWTO]], and follow the installation instructions for your platform. | You're done installing prerequisites! You can now go back to the [[GBrowse 2.0 Install HOWTO]], and follow the installation instructions for your platform. | ||
+ | |||
+ | == Installing BioPerl == | ||
+ | |||
+ | GBrowse versions 2.27 and higher require BioPerl 1.069, which has not been released as of 1 March 2011. To install BioPerl from source, do: | ||
+ | |||
+ | wget --no-check-certificate \ | ||
+ | http://github.com/bioperl/bioperl-live/tarball/master -O bioperl.tar.gz | ||
+ | tar zxvf bioperl.tar.gz | ||
+ | cd bioperl-bioperl* | ||
+ | perl Build.PL | ||
+ | ./Build test | ||
+ | sudo ./Build install | ||
+ | |||
+ | |||
+ | You're done installing prerequisites! You can now go back to the [[GBrowse 2.0 Install HOWTO]] and follow the installation instructions for your platform. | ||
=MacOSX= | =MacOSX= |
Revision as of 02:25, 1 March 2011
Before installing GBrowse 2.0, you will need to install some prerequisites on your system. Most prerequisites come as prepackaged binaries which can be installed from the command line or via GUI tools. This document describes the prerequisites for Linux, Mac OSX, and Microsoft Windows systems.
Contents
Linux systems
The following prerequisites are needed to run GBrowse on Linux systems:
Applications and Libraries
- The Apache2 web server
- Perl 5.8 or higher
- The libgd2 library
Although not required for basic operation, you will probably want at least one relational database backend. One or both of:
Those who want to obtain the best performance should consider the mod_perl, or FastCGI Apache modules:
Those who wish to generate publication-ready PDFs from GBrowse tracks should install the inkscape package:
These applications can be installed from source code or from binary packages using one of the binary packaging systems described below.
Perl Modules
GBrowse depends on the following standard Perl libraries:
- Module::Build
- GD
- Bio::Perl (version 1.6.0 or higher)
- Bio::Graphics
- JSON
- LWP
- Storable
- IO::String
- Capture::Tiny
- File::Temp
- Digest::MD5
- CGI::Session
- Statistics::Descriptive
In addition, the following optional Perl libraries are recommended:
- GD::SVG (for PDFs and editable high-resolution images)
- DBI (for relational database access)
- DBD::mysql (for MySQL database backends)
- DBD::Pg (for PostgreSQL database access)
- DB_File::Lock (for load balancing in rendering slavesstrongly)
- File::NFSLock (if two or more servers are sharing the same NFS-mounted directory for sessions)
- Template (to run the population allele frequency visualization demo)
It is easiest to use the Perl CPAN shell to install these modules. If you use the CPAN shell to install GBrowse, then the required modules will be installed automatically, however the optional ones will still need to be installed by hand:
% perl -MCPAN -e shell cpan shell -- CPAN exploration and modules installation (v1.9301) cpan[2]> install GD::SVG cpan[3]> install DBI cpan[4]> install DB_File::Lock ...etc...
Please install libgd2 and your distribution's compiler tools (e.g. "libc6-dev" on Debian systems) before you attempt to install Bio::Graphics::Browser from the CPAN shell. Otherwise it will fail at the GD library installation step.
Binary Packages
If you use a modern Linux distribution, most prerequisites are available as binary packages. There are two common package managers, Debian (DEB) and RedHat Package Manager (RPM). The next section shows you how to use command-line package management tools to install the prerequisites.
DEB Systems (apt)
Distributions on which apt is installed by default include Ubuntu (and its variants), Debian, and Mint. To install all prerequisites except for BioPerl, run the following commands as the root or using sudo (comments begin with the # sign). Some of these packages may already be installed on your system.
#Utilities to help with fetching components distributed in source code sudo apt-get install wget subversion libc6-dev build-essential #apache2 with acceleration sudo apt-get install apache2 libapache2-mod-fcgid libapache2-mod-perl2 #fast lightweight database for managing user accounts sudo apt-get install sqlite3 libdbd-sqlite3-perl #Perl libraries sudo apt-get install libmodule-build-perl libgd-gd2-perl libio-string-perl \ libcapture-tiny-perl libcgi-session-perl libwww-perl libstatistics-descriptive-perl \ libjson-perl libjson-any-perl libsvg-perl perl-doc libtemplate-perl libgd-svg-perl # optional libraries for using MySQL and PostgreSQL backends sudo apt-get install mysql-server mysql-client libdbd-mysql-perl libmysqlclient-dev postgresql libdbd-pg-perl # optional libraries for e-mail-based user registration and OpenID logins sudo apt-get install libdigest-sha-perl libssl-dev libmath-bigint-gmp-perl libnet-openid-consumer-perl libnet-smtp-ssl-perl libauthen-sasl-perl
All these installation steps must be executed as root (shown here using sudo).
RPM Systems (yum)
This includes Fedora, RedHat, SuSE, Mandriva, PCLinuxOS, and CentOS. Most of these distributions have the command line package management tool yum which is used in the following command examples. These commands will need to be run as root (or equivalently by using sudo in the manner shown above):
#Utilities to help with fetching components distributed in source code yum install wget yum install cvs yum install subversion #apache2 yum install httpd #libgd2 and its Perl library yum install gd-devel yum install perl-GD #other Perl modules yum install perl-Module-Build yum install perl-CPAN yum install perl-IO-String yum install perl-Capture-Tiny yum install perl-CGI-Session yum install perl-JSON perl-JSON-Any yum install perl-libwww-perl #optionally... yum install mysql-server mysql-libs perl-DBD-MySQL yum install postgresql postgresql-server perl-DBD-Pg yum install mod_perl yum install mod_fcgid yum install fcgi-perl yum install inkscape yum install perl-GD-SVG
If you plan to use the CPAN shell to install GBrowse, then you are all done installing prerequisites, and can return to the GBrowse 2.0 Install HOWTO. Otherwise, you should first install Bio::Perl and Bio::Graphics using the CPAN shell:
sudo perl -MCPAN -e 'install Bio::Perl' sudo perl -MCPAN -e 'install Bio::Graphics'
You're done installing prerequisites! You can now go back to the GBrowse 2.0 Install HOWTO, and follow the installation instructions for your platform.
Installing BioPerl
GBrowse versions 2.27 and higher require BioPerl 1.069, which has not been released as of 1 March 2011. To install BioPerl from source, do:
wget --no-check-certificate \ http://github.com/bioperl/bioperl-live/tarball/master -O bioperl.tar.gz tar zxvf bioperl.tar.gz cd bioperl-bioperl* perl Build.PL ./Build test sudo ./Build install
You're done installing prerequisites! You can now go back to the GBrowse 2.0 Install HOWTO and follow the installation instructions for your platform.
MacOSX
These instructions apply to Macintosh OSX version 10.5, using the fink binary installer. First, download and install Fink. Next open up the terminal window and type the following commands as the root user or with sudo (as shown):
#Utility to help components distributed in source code sudo fink install wget #libgd2 and its Perl library sudo fink install gd2 gd2-bin sudo perl -MCPAN -e 'install GD' #other Perl modules sudo perl -MCPAN -e 'install IO::String' sudo perl -MCPAN -e 'install CGI::Session' sudo perl -MCPAN -e 'install JSON' sudo perl -MCPAN -e 'install JSON::Any' #optionally. sudo fink install mysql '''more to come here''' sudo perl -MCPAN -e 'install GD::SVG'
this section is unfinished
If it isn't already activated, turn the Apache server on by activating Control Panel->Sharing->Web Sharing.